Human Phosphotyrosine EphA1 PharmaGenie ELISA Kit (SBRS1805)

The Assay Genie phosphotyrosine EphA1 immunoassay quantitatively measures activated receptor tyrosine kinase in cell lysates and tissue lysates prepared from human samples. EphA1 belongs to the ephrin receptor subfamily of receptor tyrosine kinases and is implicated in developmental patterning, cell migration, and several solid tumour contexts, making phospho-EphA1 readout useful for studies of receptor autophosphorylation, downstream signalling cascades, and drug-response profiling in cancer cell models.

This 96-assay microplate format relies on a phosphotyrosine-specific capture approach combined with a human-reactive antibody pair, providing human-specific detection without significant cross-reactivity with non-human ephrin receptors. Researchers can pair the kit with cell stimulation experiments using ephrin-A ligands or tyrosine kinase inhibitors to follow changes in EphA1 phosphorylation state across stimulation time points and dose ranges.

Each kit ships with pre-coated microwell strips and all reagents needed to run the assay, including diluted standards, detection antibody, and wash buffer. Store reagents refrigerated at 4 degrees C upon arrival, and avoid repeated freeze-thaw cycles of component vials to preserve antibody activity through the stated shelf life. The assay is supplied for research use only and is intended for in vitro phosphorylation studies of EphA1 in lysate-based workflows.