Human Phospholipid Scramblase 2/PLSCR2 ELISA Kit

Assay Genie offers a Sandwich ELISA (Double Antibody) kit designed for the quantitative measurement of Phospholipid Scramblase 2 (PLSCR2) in human samples. The assay is intended for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and uses a colorimetric detection format with a TMB substrate read at 450 nm. PLSCR2 is a protein involved in phospholipid scrambling, a process important for cell membrane maintenance and regulation, and its dysregulation has been linked to conditions including cancer, cardiovascular diseases, and autoimmune disorders.

Performance characteristics quoted from the manufacturer's product specification include a sensitivity of 0.188 ng/mL and a working range of 0.313-20 ng/mL. The kit is shipped on ice packs and components are stored at 2-8°C, with selected components additionally suitable for 12 months at -20°C. The workflow uses a biotin-labelled detection antibody followed by HRP-Streptavidin (SABC), with primary incubation at 37°C for 90 minutes, detection antibody binding for 60 minutes, SABC binding for 30 minutes, and TMB colour development for 10-20 minutes in the dark.

Supplied reagents include a pre-coated dismountable ELISA microplate, lyophilized standard, biotin-labelled antibody (100X concentrate), HRP-Streptavidin conjugate (SABC, 100X), TMB substrate, sample dilution buffer, antibody dilution buffer, SABC dilution buffer, stop solution, wash buffer (25X), and plate sealer. The associated Uniprot reference for the target is Q9NRY7. Additional reagents required but not provided include a 37°C incubator, a plate reader with a 450 nm filter, precision pipettes, distilled water, disposable tubes, and absorbent paper.