Human Phospholipase D2 (PLD2) ELISA Kit

Phospholipase D2 (PLD2) is an enzyme involved in lipid metabolism and signalling pathways, with established roles in cell growth, proliferation, and membrane trafficking. Dysregulation of PLD2 has been implicated in cancer, inflammatory disorders, and neurodegenerative conditions, making it a valuable biomarker for research into disease mechanisms and potential therapeutic interventions. This Assay Genie Sandwich ELISA Kit is designed for the quantitative detection of human Phospholipase D2 levels.

The assay is reactive with human samples and accepts serum, plasma, tissue homogenates, cell culture supernatants, and other biological fluids. It is highly specific for natural and recombinant human PLD2. Detection is colorimetric, read at 450 nm. Performance specifications include a detection range of 0.78-50 ng/mL, a sensitivity of 0.31 ng/mL, an intra-assay CV of 5.1%, and an inter-assay CV of 8.3%. Linearity was demonstrated at 1:2 to 1:16 dilutions: serum spanned 83-115%, EDTA plasma 86-119%, and heparin plasma 82-117%. Recovery averaged 100% (94-106%) for serum and 102% (96-108%) for plasma.

PLD2 is also known as Choline phosphatase 2, PLD1C, and Phosphatidylcholine-hydrolyzing phospholipase D2. The protein localises to the peripheral membrane, may function in signal-induced cytoskeletal regulation and/or endocytosis, and interacts with EGFR and PIP5K1B. Listed research area: cardiovascular. For research use only.