Human Phospholipase D (PLD) ELISA Kit

For research laboratories studying phospholipid metabolism and cell signaling, Assay Genie supplies a quantitative immunoassay kit configured to measure human Phospholipase D in experimental specimens. The PLD enzyme hydrolyzes phosphatidylcholine to generate phosphatidic acid and choline, two lipid second messengers central to membrane trafficking, cytoskeletal reorganization, and cell proliferation pathways. This microplate-based kit supports studies of PLD1 and PLD2 isoform activity across oncology, inflammation, and neurodegeneration research programs.

Each box contains a pre-coated microplate, lyophilized calibration standards, detection antibody, chromogenic substrate solution, wash buffer, and a printed protocol insert suitable for routine bench-top use. The assay incorporates a reporting step that produces an absorbance change proportional to PLD concentration in the loaded sample, read on a standard laboratory microplate reader. Researchers can apply the kit to cell lysates, tissue homogenates, and conditioned media from cell culture experiments, and pair the resulting data with downstream western blot or flow cytometry work.

Refrigerate the kit between uses and protect light-sensitive components from light as indicated on the component labels. The complete kit format supports in vitro signal transduction research, and the assay kit is intended for research use only, not for diagnostic or clinical decision-making.