Human Phospholipase C Gamma 2, Phosphatidylinositol Specific (PLCg2) ELISA Kit

PLCg2 catalyzes hydrolysis of phosphatidylinositol 4,5-bisphosphate to generate inositol 1,4,5-trisphosphate and diacylglycerol, mediating intracellular calcium release and protein kinase C activation downstream of immune receptor and receptor tyrosine kinase signaling. This Assay Genie kit enables quantitative measurement of PLCg2 protein in cell lysates and tissue homogenates for research applications including B-cell receptor signaling studies, investigation of PLCg2 gain-of-function variants linked to PLAID and APLAID autoinflammatory syndromes, platelet activation research, and osteoclast differentiation biology. Species reactivity has been validated for human PLCg2, with a recommended sample volume of 100 microliters per well for cell lysate and tissue homogenate preparations.

The format includes pre-coated microplate wells, capture and detection antibodies, recombinant standards, and assay buffer reagents. Store unopened components refrigerated at 4 degrees C, protected from light, and avoid repeated freeze-thaw cycles of the standards. Intended for research use only.