Human Phospholipase A2, Lipoprotein Associated (LpPLA2) ELISA Kit

Lipoprotein-associated phospholipase A2 (Lp-PLA2) is a calcium-independent serine lipase secreted primarily by inflammatory cells, and elevated circulating concentrations serve as an independent predictor of cardiovascular events and atherosclerotic plaque vulnerability. This Assay Genie kit enables quantification of Lp-PLA2 in human biofluid specimens, supporting investigations into inflammatory pathways of vascular disease, plaque biology, and lipid-mediated oxidative injury.

The 96-assay format includes a pre-coated microplate, biotinylated detection antibody, standard calibrators, and substrate solution suitable for absorbance-based readout. Each kit contains reagents sufficient for one complete plate run, with individual vials of standards and detection reagents for reproducible standard curve generation. Recommended storage is refrigerated at standard laboratory conditions; protect from light to maintain reagent stability across multiple freeze-thaw cycles.

Standard assay workflow involves dilution of human biofluid samples, addition to pre-coated wells, incubation with the detection antibody, and absorbance measurement after substrate addition. Applications include cardiovascular biomarker discovery, clinical chemistry testing, and translational studies investigating anti-inflammatory interventions and novel therapeutic targets in atherosclerosis research.