Human Phospholipase A2, Group IVD (PLA2G4D) ELISA Kit

Researchers studying lipid mediator biosynthesis, arachidonic acid metabolism, and cutaneous inflammation can quantify human PLA2G4D protein levels using this immunoassay kit from Assay Genie. PLA2G4D is a cytosolic, calcium-dependent phospholipase A2 isoform that cleaves the sn-2 acyl bond of membrane glycerophospholipids, releasing arachidonic acid as a substrate for downstream eicosanoid production. The isoform shows enriched expression in keratinocytes and infiltrating immune cells in skin, where it has been implicated in the lipid mediator cascade underlying psoriatic inflammation, making the kit relevant to dermatology and immunology research workflows. Each kit is configured for 96 individual reactions and ships as a complete package containing a pre-coated microplate, recombinant PLA2G4D standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash concentrate, TMB substrate, and stop solution for in vitro research use. The workflow follows standard biotin-streptavidin-HRP chromogenic ELISA methodology, with absorbance proportional to PLA2G4D concentration in the sample. Unopened kits should be stored refrigerated and protected from light, with all reagents equilibrated to room temperature before running the assay. The product is intended for research use only and has not been validated for diagnostic, therapeutic, or clinical decision-making.