Human Phospholipase A2 Activating Protein/PLAA ELISA Kit

Assay Genie supplies a Sandwich ELISA, Double Antibody format kit designed for the accurate and precise detection of Phospholipase A2 Activating Protein (PLAA) in human biological samples. PLAA is a key protein involved in the activation of phospholipase A2, an important enzyme in lipid metabolism and inflammatory responses, and dysregulation of PLAA has been implicated in inflammatory conditions, cardiovascular disorders, and cancer. Measuring PLAA levels can therefore provide valuable insights into disease mechanisms and potential therapeutic interventions.

The kit is intended for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and should be stored at 2-8°C for 12 months. It delivers a sensitivity of 0.188 ng/mL across a working range of 0.313-20 ng/mL. Validated recovery and linearity performance includes serum recovery ranging from 86-103% (average 96%), EDTA plasma recovery from 87-104% (average 94%), and heparin plasma recovery from 85-104% (average 93%), with sample dilutions of 1:2, 1:4 and 1:8 in serum, EDTA plasma and heparin plasma yielding linearity values between 80-101% across the conditions tested.

The assay procedure follows a stepwise workflow: equilibrate reagents and TMB substrate to room temperature and set up standards, samples and zero controls on the pre-coated plate; incubate at 37°C for 90 minutes for primary antigen binding; add biotin-labeled detection antibody and incubate at 37°C for 60 minutes; add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes; develop with TMB substrate in the dark for 10-20 minutes; then add stop solution and read absorbance at 450 nm immediately.