Human Phospholipase A1/PLA1 ELISA Kit

From Assay Genie, this Sandwich ELISA Kit enables quantitative detection of human Phospholipase A1 (PLA1), an enzyme that plays a critical role in lipid metabolism and cell signalling pathways. The kit uses a double-antibody sandwich format with a colorimetric readout and is intended for human samples including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Performance specifications include a sensitivity of 18.75 pg/mL and an assay range of 31.25-2000 pg/mL. Linearity in serum (n=5) spans 97-101% at a 1:2 dilution through 82-97% at 1:8, with EDTA plasma (n=5) ranging 90-105% to 82-100%, and heparin plasma (n=5) ranging 87-102% to 83-99%. Reported mean recoveries are 92% for serum, 97% for EDTA plasma, and 98% for heparin plasma. The kit supports research into inflammatory disorders, metabolic syndromes, and neurological conditions where dysregulation of phospholipase A1 has been implicated, and falls within the epigenetics and nuclear signaling research area.

The assay procedure involves reagent and TMB substrate equilibration, primary incubation at 37°C, biotinylated detection antibody binding, HRP-Streptavidin (SABC) binding, TMB substrate development, and absorbance measurement at 450 nm. Reagents are stored at 2-8°C for 12 months.