Human Phosphoinositide Dependent Protein Kinase 1 (PDPK1) ELISA Kit

Researchers studying AGC kinase signaling, insulin response, and tumor biology can use this immunoassay kit from Assay Genie to quantify phosphoinositide-dependent protein kinase 1 (PDPK1) protein levels in human cell lysates and tissue homogenates.

PDPK1 is a 63 kDa serine/threonine kinase that phosphorylates the activation loop of AGC family kinases, including AKT, PKC, and S6K, driving downstream pathways that govern glucose homeostasis, cell survival, and proliferation. Dysregulated PDPK1 activity is implicated in metabolic disease, multiple cancers, and chronic inflammatory conditions, where reliable protein quantification matters for both basic discovery and translational work.

Supplied as a complete microplate-based system with pre-coated wells, detection antibodies, standards, and assay buffer, the kit provides sufficient reagents for 96 determinations. Each well accommodates a standard sample volume, supporting parallel measurement of treated and untreated controls, time-course experiments, or dose-response screens in a single run. Validated for human reactivity, the kit supports workflows including validation of siRNA or CRISPR knockdowns, profiling of patient-derived tumor samples, and correlation of kinase expression with disease phenotype.