Human Phosphoglycerate kinase 1 (PGK1) ELISA Kit

Designed for the quantitative detection of Phosphoglycerate kinase 1 (PGK1) in human serum, plasma and cell culture supernatants, this Sandwich ELISA from Assay Genie offers high sensitivity and specificity for accurate, reproducible results. PGK1 is a glycolytic enzyme that catalyses the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, and dysregulation of the protein has been implicated in cancer and metabolic disorders, making it a key biomarker for related research.

The kit is reactive with human samples and accepts serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. Specificity is established for natural and recombinant human PGK1. Reported detection range is 0.312-20ng/mL with a sensitivity of 0.163ng/ml. Intra-assay CV is 6.0% and inter-assay CV is 10.4%. Linearity has been characterised across serum, EDTA plasma and heparin plasma dilution series, with recovery ranges of 83-95% for serum and 85-97% for plasma.

Annotated synonyms for the target include Cell migration-inducing gene 10 protein, Primer recognition protein 2, PRP 2, MIG10, OK/SW-cl.110 and PGKA. The protein is a monomer located in the cytoplasm (UniProt P00558). For research use only.