Human Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) ELISA Kit

The Assay Genie quantitative immunoassay kit enables sensitive measurement of human Phosphoenolpyruvate Carboxykinase 1 (PCK1) protein in human plasma, serum, and tissue homogenate preparations. PCK1 encodes the cytosolic isoform of phosphoenolpyruvate carboxykinase, the rate-limiting enzyme that converts oxaloacetate into phosphoenolpyruvate during gluconeogenesis. By quantifying circulating and tissue PCK1 levels, this kit supports research into hepatic glucose output, fasting metabolism, insulin resistance, non-alcoholic fatty liver disease, and the reprogrammed energy balance observed in obesity and certain tumor microenvironments.

Built on a microplate immunoassay workflow, the kit includes pre-coated wells, assay diluent, detection reagents, wash buffer, substrate solution, stop solution, and a recombinant human PCK1 standard curve sufficient for 96 assays in a single run. The protocol is suitable for in vitro research use only and is compatible with standard laboratory plate readers capable of absorbance measurement. Researchers studying gluconeogenic enzyme regulation, endocrinology, and metabolic disease pathways will find this kit useful for expression profiling, comparison with the mitochondrial PEPCK-M (PCK2) isoform, and longitudinal sample monitoring.