Human Phospho-STAT6 (Y641) PharmaGenie ELISA Kit (SBRS1994)

Quantify tyrosine-phosphorylated STAT6 at residue Y641 in human samples with this Assay Genie cell signaling ELISA. STAT6 activation downstream of IL-4 and IL-13 receptor signaling is a hallmark of Th2 immune responses, IgE class-switch recombination, allergic inflammation, and certain tumor survival pathways, making phospho-STAT6 a widely studied marker in immunology and oncology research.

The kit is configured for 96 assays using human cell lysates and tissue lysates as input material. It applies a phospho-specific capture antibody that recognizes the Y641 epitope and produces an absorbance-based colorimetric readout on a standard microplate reader. Researchers commonly stimulate cultured cells with IL-4, IL-13, or pathway inhibitors before lysis to compare activation-state changes across treatment conditions.

Assay Genie supplies a pre-coated plate, detection antibody, standard dilution series, wash buffer, substrate, and stop solution. The protocol is compatible with mammalian cell culture lysates prepared in standard RIPA or detergent-based buffers. Store kit components refrigerated, protect the substrate from light, and limit freeze-thaw cycles of the antibody reagents to preserve shelf life and lot-to-lot consistency. The kit is intended for research use only and supports studies of asthma, parasite immunity, B-cell maturation, and STAT6-driven tumor biology.