Human Phospho-STAT5A (Y694) and Total STAT5A PharmaGenie ELISA Kit (SBRS1991)

The Phospho-STAT5A (Y694) and Total STAT5A PharmaGenie ELISA Kit from Assay Genie enables simultaneous quantification of tyrosine 694-phosphorylated STAT5A and total STAT5A protein within the same sample, supporting normalization of pathway activation state to total expression. STAT5A is a critical mediator of cytokine-driven JAK-STAT signaling, activated downstream of JAK1, JAK2, and JAK3 by ligands including IL-2, IL-3, IL-5, IL-7, IL-15, GM-CSF, erythropoietin, thrombopoietin, growth hormone, and prolactin. Researchers working in hematopoiesis, NK cell biology, T-cell development, and cancer signaling studies use this ELISA to profile pathway engagement in cell lysates and tissue lysates. The microplate-based readout pairs phospho-specific and pan-STAT5A capture antibodies, enabling activation ratios to be derived from a standard curve generated with recombinant protein standards. For sample preparation, cells or tissue should be lysed in the presence of phosphatase inhibitors to preserve the labile Y694 phospho-epitope. Aliquot reconstituted standards, store at the temperature indicated on the product insert, and limit freeze-thaw cycles to maintain consistent quantification across runs.