Human Phospho-STAT2 (Y689) and Total STAT2 PharmaGenie ELISA Kit (SBRS1985)

The Phospho-STAT2 (Y689) / Total STAT2 assay quantitatively measures tyrosine 689 phosphorylation of STAT2 alongside total STAT2 protein in a single workflow, allowing researchers to normalize activation state against total expression across treatment conditions.

STAT2 is a key effector of the type I interferon (IFN-alpha/beta) JAK-STAT signaling cascade and, together with STAT1 and IRF9, forms the ISGF3 transcription complex that drives antiviral gene expression in human cells. Monitoring phosphorylation at Y689 is widely used in innate immune activation studies, viral response investigations, and cytokine signaling research using cell and tissue lysates.

Assay Genie's dual-target plate-based format supports parallel capture of phospho- and total-protein within the same run, improving data interpretation across experimental conditions. Reagents should be stored according to the product insert; avoid repeated freeze-thaw cycles of antibody components to preserve binding activity. For research use only; not for diagnostic or clinical procedures.