Human Phospho-SRC (Y419) PharmaGenie ELISA Kit (SBRS1977)

By targeting the Y419 autophosphorylation site, this Phospho-SRC immunoassay from Assay Genie quantifies the catalytically active form of SRC kinase in cell and tissue lysates, supporting pathway studies in oncology and signal transduction research. The microplate-based format allows side-by-side comparison of stimulated versus resting samples, enabling quantitative analysis of growth-factor, integrin, or kinase-inhibitor effects on SRC activation. Each kit provides reagents for 96 determinations with a recommended sample volume of 100 µL per well, and is intended for research use only in the in vitro study of human SRC phosphorylation dynamics. Pair the phospho-specific read-out with total SRC normalization to interpret activation ratios across different treatment conditions, including dose-response and time-course experiments probing upstream receptor activity.