Human Phospho-SMAD5 (SER463/465) PharmaGenie ELISA Kit (SBRS1974)

The Human Phospho-SMAD5 (SER463/465) PharmaGenie ELISA Kit from Assay Genie is a phosphorylation-specific immunoassay developed for detection of human SMAD5, quantifying the active, receptor-phosphorylated form of SMAD5 in human cell lysates and tissue lysates with reactivity specific to the human target. SMAD5 is a receptor-regulated SMAD (R-SMAD) that becomes phosphorylated at its C-terminal Ser463/465 residues following activation of BMP type I receptors, making this phospho-epitope a direct biochemical readout of canonical BMP pathway activity. Researchers apply the kit to track BMP-induced signaling dynamics in osteoblast differentiation, chondrogenesis, hematopoietic stem cell regulation, and neuronal development models, as well as to profile dysregulated BMP responses in fibrotic, inflammatory, and oncologic settings. The assay is supplied as a complete 96-assay microplate format with all reagents required for sample processing and phospho-SMAD5 detection in research workflows.