Human Phospho-RSK1 (S380) PharmaGenie ELISA Kit (SBRS1955)

The Phospho-RSK1 (S380) PharmaGenie ELISA Kit from Assay Genie specifically quantifies phosphorylation of ribosomal protein S6 kinase alpha-1 (RPS6KA1) at serine 380, a critical autophosphorylation site within the C-terminal kinase domain. ERK1/2-mediated phosphorylation at this residue is widely used as a read-out of RSK1 catalytic activation downstream of the Ras/Raf/MEK/ERK cascade, making the microplate-based assay valuable for cell signaling studies, cancer research, MAPK pathway inhibitor screening, and investigations of growth factor or mitogen responses. Compatible with cell lysate and tissue lysate samples, the kit supports in vitro workflows examining upstream kinase activity, feedback regulation, and crosstalk between the MAPK and mTOR signaling pathways.