Human Phospho-RSK1 (S380) and Total RSK1 PharmaGenie ELISA Kit (SBRS1956)

This dual-analyte immunoassay from Assay Genie enables parallel quantification of RSK1 phosphorylated at the activating serine 380 residue and total RSK1 protein from a single lysate sample, supporting direct normalization of phosphorylation status to total expression levels. RSK1, also called p90RSK or MAPKAP-K1, sits downstream of the Raf/MEK/ERK cascade, and S380 autophosphorylation reflects full catalytic activation following ERK-mediated priming phosphorylation. The kit supports cell signalling studies of growth factor responses, oncogenic pathway activation, and small-molecule kinase inhibitor profiling in cancer and neurobiology research. Lysates should be prepared in RIPA buffer containing fresh protease and phosphatase inhibitor cocktails to preserve the labile phospho-epitope before loading on the pre-coated microplate. Signal proportional to bound analyte is revealed through HRP-TMB enzymatic readout, providing quantitative data that complements or replaces phospho-specific western blot workflows and reduces antibody consumption. Reconstituted reagents are kept refrigerated for short-term use, while diluted standard curves should be frozen in single-use aliquots to avoid repeated freeze-thaw cycles and preserve assay reproducibility.