Human Phospho-Rictor (T1135) and Total Rictor PharmaGenie ELISA Kit (SBRS1949)

Phosphorylation of Rictor at threonine 1135 is a key regulatory event within the mTORC2 signaling complex, and quantifying this modification alongside total Rictor protein levels provides a direct read-out of pathway activation in research models. This dual-analyte immunoassay from Assay Genie is configured to measure both the phosphorylated T1135 epitope and total Rictor in a single workflow, supporting studies in cell signaling, cancer biology, and metabolic disease where mTORC2 activity is of interest.

Provided as a 96-assay kit on a microplate, the reagents accept cell lysates and tissue lysates as input material, making the workflow suitable for in vitro experiments using cultured mammalian cells or dissected tissue samples. Human samples and cell lines are the validated reactivity, and the assay is intended for research use only in laboratory settings exploring Rictor-driven signaling networks.

As part of the PharmaGenie ELISA portfolio, the reagents are configured to deliver parallel phospho- and total-protein measurements from the same sample set, which is useful when normalizing activation state against total expression. The plate-based format integrates with standard plate-reader instruments commonly used in research labs running ELISA workflows.