Human Phospho-PDGFR-beta (Y751) and Total PDGFR-beta PharmaGenie ELISA Kit (SBRS1927)

Phospho-PDGFR-beta (Y751) and Total PDGFR-beta PharmaGenie ELISA Kit from Assay Genie is a dual-target immunoassay that simultaneously quantifies the phosphorylated receptor at tyrosine 751 and the total PDGFR-beta protein within a single lysate sample. PDGFR-beta is a 190 kDa receptor tyrosine kinase whose phosphorylation at Y751 generates a docking site for the p85 subunit of PI3K, initiating downstream signaling through the PI3K/Akt axis that controls smooth muscle cell proliferation, pericyte recruitment, and fibrotic tissue remodeling.

The plate-based kit accepts cell lysates and tissue lysates, allowing investigators in vascular biology, oncology, and wound healing studies to normalize phosphorylation levels against total receptor expression in matched wells. Researchers typically aliquot lysates and limit freeze-thaw cycles to preserve phospho-epitope integrity before reading absorbance values to derive relative phospho/total ratios across experimental conditions.