Human Phospho-P70S6K (T421/S424)Quantitative PharmaGenie ELISA Kit (SBRS1920)

Phospho-P70S6K (T421/S424) is a key quantitative readout of mTORC1 pathway activity, downstream of PI3K and Akt signaling. The kit uses a phospho-site-specific antibody that recognizes p70 S6 kinase only when threonine 421 and serine 424 are phosphorylated, sites located in the auto-inhibitory domain whose modification is required for full kinase activation. This selectivity enables accurate comparison of basal versus stimulated signaling in cell lysates and tissue lysates. Researchers commonly apply this Assay Genie ELISA to monitor responses to growth factors, insulin, amino acids, and small-molecule mTOR pathway inhibitors, and to benchmark cancer cell line panels for differential pathway engagement. Each kit is supplied with a pre-coated microplate, phospho-specific detection antibody, lyophilized standards, buffers, and substrate; reagents should be refrigerated and protected from light. The format supports quantitative in vitro signaling studies with reproducible results across independent experiments.