Human Phospho-NBS1 (S343) and Total NBS1 PharmaGenie ELISA Kit (SBRS1910)

This Phospho-NBS1 (S343) and Total NBS1 ELISA kit from Assay Genie enables parallel measurement of NBN protein phosphorylation at Serine 343 and total NBS1 levels within the same sample, supporting normalization of DNA damage response signaling readouts. The microplate-based assay is intended for in vitro research applications such as studying ATM kinase activation, double-strand break repair, and genomic stability pathways. Each kit provides reagents sufficient for 96 assays, with absorbance read on a standard plate reader. Cell lysates and tissue lysates are loaded at a recommended sample volume of 100 µl per well, and components should be refrigerated upon arrival to preserve antibody activity. Researchers investigating checkpoint signaling, radiation biology, or Nijmegen breakage syndrome mechanisms can use this phospho/total ELISA to quantify pathway activation rather than relying on western blot band densitometry alone.