Human Phospho-LKB1 (Ser428) and Total LKB1 PharmaGenie ELISA Kit (SBRS1882)

A dual-analyte phosphorylation immunoassay from Assay Genie, the Human Phospho-LKB1 (Ser428) and Total LKB1 PharmaGenie ELISA Kit simultaneously quantifies LKB1 phosphorylated at serine 428 alongside total LKB1 protein in the same lysate sample, enabling direct comparison of phosphorylation status to total protein expression. LKB1, also called STK11, is a master upstream kinase that activates AMPK and a family of related kinases in response to cellular energy stress, and Ser428 phosphorylation is a critical regulatory mark controlling its activity. Researchers use this kit to compare pathway activation states between treated and untreated samples, normalize phospho-signals to total protein, and screen compounds that modulate LKB1 signaling in cancer, metabolic disease, and cell polarity studies. The assay accepts cell lysates and tissue lysates as input, supports studies on tumor suppressor function relevant to Peutz-Jeghers syndrome and non-small cell lung cancer, and pairs well with western blot confirmation experiments. Each kit provides reagents for 96 assays and includes the proprietary PharmaGenie protocol optimized for reproducible signal quantification across a standard microplate workflow.