Human Phospho-LCK (Y394) PharmaGenie ELISA Kit (SBRS1879)

The Assay Genie Phospho-LCK (Y394) PharmaGenie ELISA enables quantitative measurement of tyrosine 394 phosphorylation on human LCK, a Src-family kinase central to T-cell receptor signaling. The assay uses a pair of antibodies selective for the phosphorylated activation loop, allowing researchers to distinguish active kinase from total LCK in cell lysates and tissue lysates. Each kit provides reagents for 96 assays in a microplate format. The capture antibody is human-specific, and the reagents are validated for use with cultured cell extracts and tumor tissue homogenates. Researchers studying T-cell activation, immune signaling, leukemia biology, and Src-family kinase pathway regulation can use this phosphorylation ELISA to monitor LCK activity under drug treatment, stimulation, or gene knockdown conditions. Store kit components refrigerated; avoid repeated freeze-thaw cycles of antibody and standard reagents.