Human Phospho-LAT (Y161) PharmaGenie ELISA Kit (SBRS1876)

Phosphorylation of the linker protein LAT at tyrosine 161 is an early biochemical event during T cell receptor engagement, where Src-family and ZAP-70 kinases modify this adapter to assemble the downstream signaling complex at the plasma membrane. The Human Phospho-LAT (Y161) PharmaGenie ELISA Kit from Assay Genie is a phosphorylation-specific immunoassay that quantifies this discrete phospho-epitope in lysates prepared from human immune cells or lymphoid tissue.

Configured for 96 assays on a standard microplate, the kit accepts cell lysate and tissue lysate inputs and produces a chromogenic read-out that any standard plate reader can record. Immunology and signal-transduction laboratories apply the assay to monitor T cell activation state, evaluate small-molecule kinase inhibitor pharmacology, and compare phospho-LAT Y161 levels between resting and stimulated donor samples.

Because the capture is selective for the phosphorylated Y161 residue rather than total LAT protein abundance, the kit is well matched to studies where transient kinase activity, not steady-state expression, is the variable of interest. All reagents required for one full microplate run are supplied, and the product is intended for research use only with human specimens.