Human Phospho-IRF-3 (S386) PharmaGenie ELISA Kit (SBRS1863)

The Human Phospho-IRF-3 (S386) PharmaGenie ELISA Kit from Assay Genie quantitatively measures interferon regulatory factor 3 phosphorylated at serine 386, a post-translational modification catalyzed by TBK1 and IKKε kinases during activation of the RIG-I/MDA5 and TLR3 antiviral pathways. Researchers use this phosphorylation ELISA to monitor type I interferon signaling, innate immune responses to viral infection, and pattern recognition receptor cascades in stimulated cell culture models and primary tissue specimens, with each sample well loaded using 100 µL of prepared lysate. The kit supplies a complete set of pre-coated microplate wells, lyophilized standards, detection antibody, wash buffer, substrate solution, and stop reagent, supporting measurement of phospho-IRF-3 (S386) levels in cell lysates and tissue lysates prepared under phosphatase-inhibitor conditions. Each microplate provides 96 assays in a single-use, break-apart well format appropriate for studies requiring small to medium sample cohorts. Reagent components should be stored refrigerated at 4 °C to preserve antibody activity, with the reconstituted standard kept frozen at -20 °C in single-use aliquots to limit freeze-thaw cycles. Detection is read on a standard plate reader, and the assay is validated for research use only in human samples, offering a quantitative alternative to phospho-specific western blot workflows for tracking IRF-3 pathway activation.