Human Phospho-INSR (Y1189) PharmaGenie ELISA Kit (SBRS1860)

Insulin receptor signaling is central to glucose homeostasis, and phosphorylation of tyrosine 1189 within the kinase activation loop is a widely used biomarker of receptor engagement. The Phospho-INSR (Y1189) PharmaGenie ELISA Kit from Assay Genie is a phosphorylation-specific immunoassay that quantitatively measures this site in human cell lysates and tissue lysates, supporting research into insulin action, metabolic disease mechanisms, and receptor tyrosine kinase biology.

Each kit provides reagents sufficient for 96 assays in a microplate format, with all buffers, standards, and detection components supplied for direct laboratory use. The assay uses a colorimetric readout, allowing quantification on a conventional plate reader without specialized instrumentation. Because the antibodies are phospho-specific at Y1189, the kit enables discrimination between activated and basal states of the insulin receptor in response to ligand stimulation, kinase inhibitors, or downstream signaling perturbations.

Typical applications include profiling insulin receptor activation in cultured cell lines, characterizing insulin signaling dynamics in primary tissue samples, and supporting drug-discovery programs targeting metabolic disorders such as type 2 diabetes. Samples are prepared by lysing cells or tissues under conditions that preserve phosphorylation, and the resulting lysates are added directly to the pre-coated plate wells following the provided protocol.

Storage recommendations call for keeping the kit refrigerated between uses, with individual component instructions specified in the product insert. The complete protocol, reagent preparation steps, and data analysis guidelines are included to support reproducible quantification of phospho-INSR Y1189 across experiments.