Human Phospho-INSR (Y1189) and Total INSR PharmaGenie ELISA Kit (SBRS1861)

The Phospho-INSR (Y1189) and Total INSR dual ELISA from Assay Genie quantitatively measures insulin receptor autophosphorylation alongside total receptor levels in a single experiment. This pairing supports normalization to receptor expression, eliminating confounding from sample-to-sample variation in INSR abundance across diabetes, metabolic syndrome, and cancer biology studies where aberrant insulin signaling plays a pathophysiological role.

Tyrosine 1189 resides within the kinase activation loop of the insulin receptor beta subunit, and phosphorylation at this site correlates directly with receptor activation following insulin binding. Tracking phospho-INSR together with total INSR over time-course or dose-response experiments with insulin or IGF-1 stimulation enables researchers to distinguish true changes in receptor activation from fluctuations in expression.

Lysate preparation should include phosphatase inhibitor cocktails to preserve the Y1189 phosphorylation signal during handling. The kit supports investigation of small-molecule kinase inhibitors, monoclonal antibody therapeutics, and gene-editing approaches targeting INSR signaling in cell culture and tissue-derived samples, with application in research use only workflows.