Human Phospho-I-kappa B alpha (S32) and Total I-kappa B alpha PharmaGenie ELISA Kit (SBRS1858)

Phospho-I-kappa B alpha (S32) and Total I-kappa B alpha are quantified in parallel from the same human sample using this dual-analyte ELISA kit from Assay Genie. IκBα functions as the cytoplasmic inhibitor of NF-κB, and phosphorylation at Ser32 targets it for proteasomal degradation, releasing NF-κB to drive transcription. Pairing the phospho-specific readout against the total-protein readout lets researchers distinguish genuine pathway activation from simple changes in inhibitor abundance.

The 96 assays configuration is validated for cell lysates and tissue lysates, supporting studies in signal transduction, inflammation biology, immune regulation, and oncology. Assay Genie supplies a complete kit with pre-coated plates, detection reagents, and standards, so laboratories do not have to source matched antibody pairs separately. Performance is optimized for human reactivity, applicable to translational work with patient-derived cells and relevant human cell lines. The colorimetric readout is captured on a conventional microplate reader.