Human Phospho-HSP27 (S82) PharmaGenie ELISA Kit (SBRS1852)

Phosphorylation-specific detection kits from Assay Genie offer targeted quantitation of post-translational modifications. This ELISA kit measures heat shock protein 27 (HSP27) phosphorylated at serine 82, a regulatory site controlled by MAPKAP kinase 2 downstream of p38 MAPK signalling. Researchers studying cellular stress responses, cytoskeletal remodelling, or kinase pathway activity use such assays to monitor activation kinetics in cultured cells and tissue preparations.

The assay is configured for 96 assays and validated for human samples, with cell lysates and tissue lysates as the recommended input. Investigators preparing lysates from treated cell lines or snap-frozen biopsy material can load the clarified supernatant directly after standard buffer-based extraction with protease and phosphatase inhibitor cocktails. Because phosphorylation is labile, prompt processing on ice and immediate snap-freezing preserve the S82 modification prior to the assay run.

Typical applications include profiling HSP27 phosphorylation following heat shock, oxidative stress, cytokine stimulation, or treatment with kinase inhibitors in cancer and inflammation research. The kit is supplied as a complete reagent set for colorimetric readout on a standard microplate reader, supporting routine screening of pathway activation across translational studies.

For research use only. Not intended for diagnostic or therapeutic procedures.