Human Phospho-HSP27 (S82) and Total HSP27 PharmaGenie ELISA Kit (SBRS1853)

Assay Genie's dual-format ELISA quantifies HSP27 phosphorylated at serine 82 together with total HSP27 in human cell lysates and tissue lysates, letting researchers compute phospho-to-total ratios from one experimental run and avoid separate loading controls. Phosphorylation at the S82 residue is a well-characterized downstream readout of p38 MAPK pathway activation via the kinase MAPKAPK2/MK2, making this kit useful for studies of cellular stress, heat shock, actin cytoskeleton remodeling, growth factor signalling and chaperone-mediated cytoprotection in cancer and inflammation research. The 96-assay kit provides coated microplate wells with reagents for sample preparation and detection; components should be refrigerated on arrival, with shelf life extending through the expiry printed on the kit label.