Human Phospho-H2AX (S139) PharmaGenie ELISA Kit (SBRS1846)

The Human Phospho-H2AX (S139) PharmaGenie ELISA Kit from Assay Genie quantitatively measures phosphorylated histone H2AX at serine 139, the established biomarker for DNA double-strand breaks (DSBs). γH2AX foci form rapidly at DSB sites and serve as a sensitive readout in genotoxicity screening, radiation biology research, cancer therapeutics development, and DNA repair pathway studies.

Built around a phosphorylation-specific antibody pair, the assay reliably detects the modified histone epitope in cell lysates and tissue lysates. Researchers use the kit to monitor ATM/ATR kinase signaling, replication stress responses, oxidative damage outcomes, and apoptosis pathways tied to genomic instability, with applications spanning in vitro cell culture models and in vivo tissue samples. The assay has been validated for human γH2AX and shows cross-reactivity with rodent and primate samples, supporting comparative DNA-damage studies across mammalian species. This phosphorylation ELISA Kit complements workflows investigating DNA-damaging agents, including chemotherapeutics, topoisomerase inhibitors, and ionizing radiation.

Each 96-well microplate kit provides reagents for quantitative ELISA detection in phosphorylation research, with a recommended sample volume of 100 µL per well. Standards, capture antibodies, and detection reagents are included, and the procedure follows a straightforward incubation and wash protocol compatible with standard microplate readers.