Human Phospho-H2AX (S139) and Total H2AX PharmaGenie ELISA Kit (SBRS1847)

This dual-target ELISA from Assay Genie enables simultaneous quantification of phosphorylated histone H2AX at Ser139 (γH2AX), an early and sensitive marker of DNA double-strand breaks, alongside total H2AX protein in human samples. The microplate format allows researchers to calculate phosphorylation stoichiometry by normalizing the phospho-H2AX signal to total H2AX within the same lysate, supporting accurate interpretation of DNA damage response (DDR) activation.

Validated for cell lysates and tissue lysates, the kit supports investigations in genotoxicity screening, radiation biology, ATM/ATR kinase signaling, homologous recombination repair, and chemotherapeutic drug response studies. Researchers can monitor DNA damage induction and repair kinetics after exposure to ionizing radiation, topoisomerase inhibitors, or other genotoxic agents, and compare baseline versus treated chromatin states.

Each plate includes recombinant protein standards calibrated for interpolation of unknown samples. Capture antibodies are specific for the phosphorylated Ser139 epitope, ensuring discrimination between damaged and undamaged chromatin. Store all components at -20 °C, avoid repeated freeze-thaw cycles, and protect antibody vials from light to preserve binding activity. Intended for research use only in academic and pharmaceutical laboratory settings.