Human Phospho-Estrogen receptor (S118) and Total Estrogen receptor PharmaGenie ELISA Kit (SBRS1814)

The Phospho-Estrogen Receptor (S118) and Total Estrogen Receptor dual ELISA Kit from Assay Genie enables researchers to quantify both phosphorylated ERα at serine 118 and total ERα protein levels from the same sample lysate, allowing direct calculation of phosphorylation stoichiometry. This is particularly valuable for breast cancer research, hormone signaling studies, and screening of selective estrogen receptor modulators (SERMs) and endocrine-disrupting compounds. The kit accommodates cell lysates and tissue lysates, with a recommended sample volume of 100 µL per well. Reagents are supplied ready-to-use in a microplate-based format, and the assay should be stored refrigerated at 4°C to preserve antibody activity. By normalizing phospho-S118 signal to total ERα, investigators can distinguish true pathway activation from changes in receptor expression alone.