Human Phospho-ErbB3 (Y1262) PharmaGenie ELISA Kit (SBRS1817)

ErbB3 (also called HER3) is a 180 kDa transmembrane receptor tyrosine kinase of the EGFR/ErbB family, and tyrosine 1262 (Y1262) is one of its major C-terminal autophosphorylation sites. Once phosphorylated, Y1262 serves as a direct docking motif for the p85 regulatory subunit of phosphatidylinositol 3-kinase, so measuring phospho-Y1262 levels is a sensitive readout of PI3K/Akt pathway activation downstream of ErbB2/ErbB3 heterodimers. This Assay Genie kit is supplied as a 96-well microplate ELISA and is used to quantify phospho-ErbB3 (Y1262) in human cell lysates and tissue lysates, supporting studies of HER3-driven tumor signaling, acquired resistance to HER2-directed therapies such as trastuzumab and lapatinib, and receptor crosstalk in breast, gastric, and ovarian cancer models. Typical input is 100 µL of clarified lysate per well. The capture antibody is raised against the human Y1262 phospho-epitope and shows no significant cross-reactivity with non-phosphorylated ErbB3 or with rodent ErbB3 orthologs, which is useful when comparing human tumor xenograft lysates to isogenic cell-line controls. For research use only.