Human Phospho-eNOS (S1177) PharmaGenie ELISA Kit (SBRS1803)

This phosphorylation ELISA from Assay Genie quantitatively measures endothelial nitric oxide synthase in its activated state at the Akt/PKB target site serine 1177, supporting research into vascular signaling, angiogenesis, shear-stress response, and endothelial function in cardiovascular and metabolic studies. The microplate assay is calibrated for cell or tissue lysate samples prepared with phosphatase inhibitors to preserve the labile S1177 modification, and the kit supplies matched capture and detection antibodies together with a phospho-eNOS standard, TMB substrate, and stop solution for absorbance-based readout. Standard kit components should be stored at -20 degrees C with the plate sealed inside its desiccated pouch, while working aliquots of buffers and antibodies remain stable through multiple freeze-thaw cycles when the assay is run for research use only.