Human Phospho-eNOS (S1177) and Total eNOS PharmaGenie ELISA Kit (SBRS1804)

From Assay Genie's Phosphorylation ELISA portfolio, this dual-analyte kit quantifies endothelial nitric oxide synthase in its activated, serine-1177-phosphorylated form alongside total eNOS protein in one experiment, enabling researchers to derive a phospho-to-total ratio as a quantitative readout of eNOS activation status. The assay is calibrated for human cell lysates and tissue lysates, supporting studies of vascular endothelial function, NO-mediated signaling, and pathways upstream of Ser1177 such as Akt, AMPK, and CaMKII. Compatible inputs include cultured endothelial lines (HUVEC, HAEC), vascular smooth muscle preparations, and snap-frozen arterial tissue homogenates. The microplate strip wells ship sealed in a foil pouch with desiccant; reconstituted standards, detection antibody, and streptavidin-HRP conjugate should be aliquoted into single-use vials and stored frozen at -20 °C to preserve activity across repeated freeze-thaw cycles.