Human Phospho-DDR2 (Tyr740) PharmaGenie ELISA Kit (SBRS1785)

Assay Genie's PharmaGenie ELISA line includes this kit for quantifying phosphorylation of Discoidin Domain Receptor 2 (DDR2) at tyrosine 740, a key autophosphorylation site within the activation loop of this collagen-binding receptor tyrosine kinase. Engagement of DDR2 by fibrillar collagen drives phosphorylation at this site, making Tyr740 a widely used quantitative readout in fibrosis research, tumor-stroma interaction studies, and wound repair signaling pathways. The kit is supplied as a complete pack with a pre-coated microplate, calibration standards, and matched detection antibodies, and is validated for cell lysates and tissue lysates prepared with standard phosphatase-inhibitor cocktails to preserve the phospho-epitope. Aliquot thawed samples and limit freeze-thaw cycles before loading, and keep working lysates on ice during assay setup. Researchers commonly pair this ELISA readout with parallel Western blot analysis of total DDR2 to normalize phosphorylation stoichiometry across treatment conditions and to control for changes in receptor expression.