Human Phospho-Cyclin B1 (S126) PharmaGenie ELISA Kit (SBRS1781)

The Phospho-Cyclin B1 (S126) ELISA Kit from Assay Genie quantitatively measures the ~48 kDa cyclin B1 protein when phosphorylated at serine 126, a regulatory site targeted by casein kinase 2 and polo-like kinase 1 during mitotic entry. Researchers studying G2/M checkpoint control, spindle assembly, and oncogenic signaling use the assay to profile kinase activity in cancer cell lines and primary tissue specimens. The kit is compatible with cell lysates and tissue lysates prepared in standard RIPA buffer, supporting workflow flexibility for studies of DNA damage response and CDK1 activation dynamics.

Each microplate-based kit contains pre-coated capture antibody, detection reagents, and calibration standards validated for human samples. The protocol requires only a small lysate volume per well, making the format efficient for sample-limited experiments. The kit is intended for research use only; store refrigerated and avoid repeated freeze-thaw cycles of reconstituted reagents to preserve antibody activity.