Human Phospho-CREB (S133) and Total CREB PharmaGenie ELISA Kit (SBRS1779)

The Assay Genie Phospho-CREB (S133) and Total CREB PharmaGenie ELISA Kit enables simultaneous quantification of phosphorylated CREB at Serine 133 alongside total CREB protein in the same lysate, supporting direct normalization of phosphorylation status to total protein expression. CREB is a calcium- and cAMP-responsive transcription factor whose activation at Ser133 is widely studied in neuronal plasticity, learning and memory, metabolic signaling, and oncology pathways including PI3K/AKT and MAPK cascades. The dual-target format is configured for cell lysates and tissue lysates, and is supplied as a complete kit with pre-coated microplate wells, capture antibodies, detection antibodies, standards, and substrate reagents, allowing researchers to monitor dynamic changes in CREB activation in response to drug treatment, growth factor stimulation, or genetic manipulation without running parallel single-analyte ELISAs. Components should be refrigerated upon receipt, and the assay is intended for research use only, not for diagnostic or therapeutic procedures.