Human Phospho-CHK2 (T68) and Total CHK2 PharmaGenie ELISA Kit (SBRS1777)

The Phospho-CHK2 (T68) and Total CHK2 dual ELISA from Assay Genie enables quantitative profiling of CHK2 activation status alongside total CHK2 protein in cell lysates and tissue lysates, supporting research into DNA damage response, cell cycle checkpoint signaling, and tumor suppressor biology. CHK2 (Checkpoint Kinase 2) is phosphorylated at threonine 68 primarily by ATM kinase in response to DNA double-strand breaks, making this site a sensitive readout of genotoxic stress, ionizing radiation, and chemotherapeutic exposure. Investigators in oncology, radiobiology, and DNA repair pathway analysis can use this microplate-based kit to monitor pathway activation kinetics, compare phospho-to-total CHK2 ratios across treatment conditions, and screen compound libraries for DNA damage response modulators. The reagents are provided for in vitro research use only, with components stored at -20 degrees C for long-term stability and protected from light where indicated.