Human Phospho-Chk1 (S280) PharmaGenie ELISA Kit (SBRS1774)

This phosphorylation ELISA from Assay Genie quantitatively measures Chk1 phosphorylated at Ser280 in human cell lysates and tissue lysates, supporting DNA damage response research and cell cycle checkpoint studies. Chk1 (Checkpoint Kinase 1) functions as a key downstream effector of ATR and is phosphorylated at S280 in connection with replication stress signaling, making this assay useful for investigations into genotoxic stress, checkpoint regulation, and kinase pathway activity. The kit employs a colorimetric read-out on a microplate format and provides 96 assays, allowing medium-throughput screening of phosphorylated Chk1 status across experimental conditions. Researchers working with human cell lines or primary tissue preparations can apply this reagent to compare treated versus untreated samples, monitor time-course phosphorylation events following DNA-damaging agent exposure, and profile signaling dynamics in cancer cell biology studies.