Human Phospho-Chk1 (S280) and Total Chk1 PharmaGenie ELISA Kit (SBRS1775)

This Phosphorylation ELISA kit from Assay Genie enables simultaneous quantification of phosphorylated Chk1 at Serine 280 alongside total Chk1 protein in the same lysate, supporting direct normalization of pathway activation against total kinase expression. Chk1 (Checkpoint Kinase 1) is a key effector of the DNA damage response and regulates the G2/M cell cycle checkpoint, so researchers measuring checkpoint signaling, replication stress, or genotoxic compound activity rely on phospho-specific readouts at sites such as S280, an autophosphorylation residue linked to kinase activity.

The kit is formulated for use with cell lysates and tissue lysates, making it appropriate for in vitro signaling studies, oncology drug screening workflows, and validation of upstream kinase or phosphatase effects on Chk1 phosphorylation status. Reconstituted reagents and the pre-coated microplate should be stored according to the package insert, typically frozen at -20 °C to preserve antibody capture performance across repeat freeze-thaw cycles.

Researchers commonly pair phospho-Chk1 measurements with western blot validation or flow cytometry-based cell cycle analysis to confirm checkpoint engagement, with the duplex format streamlining normalization without requiring separate ELISA runs for each target.