Human Phospho-C-Fos (Thr232) and Total C-Fos PharmaGenie ELISA Kit (SBRS1773)

The dual-capture format discriminates the Thr232 phosphorylated form from total C-Fos within a single microplate run, eliminating the need for parallel phospho-only and total-only experiments and allowing direct normalization of kinase pathway activity to total protein in each lysate sample. The Thr232 residue lies in the C-terminal transactivation domain, a region whose modification gates dimerization with c-Jun and subsequent AP-1-driven transcription, so quantitative measurement of site occupancy is informative for studies of growth factor signaling, stress responses, and immediate-early gene induction in cell lysates and tissue lysates. Standard curves from Assay Genie span the working range of the format, with sample volumes of 100 uL loaded per well according to the supplied protocol, and the kit is configured for research use only in vitro laboratory workflows.