Human Phospho-BTK (Y551) and Total BTK PharmaGenie ELISA Kit (SBRS1767)

The Human Phospho-BTK (Y551) and Total BTK PharmaGenie ELISA from Assay Genie enables simultaneous measurement of BTK phosphorylated at tyrosine 551 alongside total BTK protein in the same sample, supporting accurate normalization of phosphorylation events. BTK (Bruton's tyrosine kinase) is a key mediator of B-cell receptor signaling, and the Y551 autophosphorylation site in its kinase domain is a critical readout of BTK activation. Researchers use this dual-detection format to evaluate BTK pathway activity in response to BCR stimulation and to profile the cellular effects of covalent and non-covalent BTK inhibitors such as ibrutinib and acalabrutinib in lymphoma, leukemia, and autoimmune disease models.

The kit accepts cell and tissue lysates prepared with standard phosphatase-compatible buffers; 100 µL of lysate is loaded per well in the supplied microplate. Antibody pairs are pre-validated to recognize the activated Y551 phospho-epitope and a conserved BTK region, allowing direct comparison of phosphorylated and total protein levels without running parallel assays. Intended for research use only, with components stored at -20 °C and protected from light to preserve antibody activity across freeze-thaw cycles.