Human Phospho-BAD (S112) PharmaGenie ELISA Kit (SBRS1759)

This phosphorylation-specific immunoassay from Assay Genie quantitatively measures BAD protein phosphorylated at serine 112 in human cell lysates and tissue lysates. BAD is a BH3-only member of the Bcl-2 family and a central regulator of the mitochondrial apoptosis pathway; phosphorylation at S112 by survival kinases such as Akt/PKB and PKA disrupts its interaction with anti-apoptotic Bcl-2 proteins, making this phospho-epitope a widely used readout in cancer biology, neurodegeneration studies, and small-molecule drug screening programs aimed at modulating survival signaling. Supplied as a complete 96-assay microplate kit, the format includes coated wells, detection antibodies, buffers, and substrate sufficient for running full signaling experiments. A colorimetric read-out enables straightforward analysis on standard plate readers without specialized instrumentation. Researchers investigating PI3K/Akt pathway activity, chemoresistance mechanisms, or apoptosis signaling will find this ELISA useful for time-course and dose-response studies using lysates prepared from cultured mammalian cells or snap-frozen tissue specimens. The kit is intended for research use only and is configured specifically for human samples.