Human Phospho-BAD (S112) and Total BAD PharmaGenie ELISA Kit (SBRS1760)

Measure phosphorylation of BAD at serine 112 alongside total BAD protein levels in human samples with this dual-analyte immunoassay from Assay Genie. The kit pairs capture antibodies specific for the phospho-S112 epitope and the full-length BAD protein, allowing researchers to quantify activation-state changes in the apoptosis pathway using the same lysate. Both targets are reported on a single standard curve set, which simplifies normalization when comparing treatment conditions.

Validated for cell lysates and tissue lysates, the assay uses a colorimetric readout compatible with standard microplate readers. Each kit contains strips, diluents, and reagents sufficient for 96 assays, supporting mid-throughput screening campaigns in cancer biology, kinase inhibitor profiling, and survival signaling studies. Researchers working on BAD-dependent apoptotic regulation, PI3K/Akt pathway modulation, or mitochondrial stress responses will find the phospho/total ratio useful for time-course and dose-response experiments.

The kit is intended for research use only and is reactive with human samples. Store reagents refrigerated and protect from light to preserve antibody activity through the full workflow.