Human Phospho-AXL (Y779) and Total AXL PharmaGenie ELISA Kit (SBRS1758)

The Human Phospho-AXL (Y779) and Total AXL PharmaGenie ELISA Kit from Assay Genie quantifies tyrosine-phosphorylated AXL at residue Y779 alongside total AXL protein on a pre-coated microplate. The dual-analyte workflow lets researchers normalize phosphorylation signal to receptor abundance in the same well, which is important when comparing activation states between treated and untreated samples. Y779 sits within the AXL kinase activation loop, a known autophosphorylation site widely used as a read-out of TAM receptor activity in cell signalling research.

The kit is validated for cell lysates and tissue lysates prepared with standard lysis buffers containing phosphatase inhibitors to preserve phosphorylation at residue 779. Investigators working on GAS6/TAM signalling, tumour invasion, or drug-response profiling can monitor pathway engagement after ligand stimulation or small-molecule inhibition. Reagents are supplied ready-to-use, with matched detection antibodies distinguishing the phosphorylated form from total receptor.

Keep the kit refrigerated on arrival; reconstituted standards should be aliquoted to limit freeze-thaw cycles. The microplate supports up to 96 assays in a single run.