Human Phospho-AKT (S473)Quantitative PharmaGenie ELISA Kit (SBRS1744)

This phosphorylation ELISA from Assay Genie specifically quantifies AKT (Protein Kinase B) when activated at serine 473, the critical regulatory site governing downstream mTOR signaling, cell survival, and metabolic responses. Investigators studying the PI3K/AKT/mTOR axis apply this kit in oncology, insulin resistance, and neurodegeneration workflows to monitor pathway activation following stimulation or pharmacological inhibition across human, mouse, and rat samples.

Prepared cell lysates and tissue lysates supplemented with phosphatase inhibitor cocktails preserve the S473 phosphate group, and the assay delivers quantitative readouts via a colorimetric endpoint measured on a standard microplate reader. Refrigerate all kit components at 4 degrees C upon arrival and limit freeze-thaw cycles of the standard and detection antibody to maintain calibration. For research use only.